• Quick summary of topics:

    Terminology
    pipetting techniques

    Low volume dry plate pipetting.
    · Volume calibration.
    · Setting up liquid classes.
    · How to reduce liquid retention in tips when using the same tips for
    multiple plates.
    · Multi-pipetting (e.g. take up 15µl and dispense 5µl to all plates
    accurately).
    · How to mix properly after pipetting

    Wash Cycles
    Dilution Effects with Buffers
    reagent and chemical compatibility

    Overview of Validation and QC
    Color Dyes

    Decontamination

    Goals of the course:

    • Familiarize you with the terminology and methods of automated liquid handling
    • Discuss “optimal” pipetting techniques
    • Learn about reagent and chemical compatibility issues
    • Introduction to validation/QC testing options
    • Review proper decontamination techniques
    • Try hands-on operations on real liquid handling robots with an aqueous liquid class using a calibration kit modifying aspiration and dispense speeds with contact and non-contact dispensing handling “issues” (the majority of the class)!

    Full Summary of Course Topics:

    1.Introduction to liquid handling robots for HTS
    a.Fixed tips versus disposable tips
    2.Liquid handling terminology, issues and techniques
    3.Reagent and chemical compatibility and handling issues
    4.Dilution Effects in Assay Development
    5.Validation and QC techniques and options
    6.Decontamination protocols
    7.Hands-on pipetting on real robots modifying speed in delivering a Standard aqueous liquid class solution colored with scientific dye(s)
    a)Measuring CV precision
    b)Measuring CV accuracy
    c)Varying dispensing speed
    d)Varying Aspiration
    e)For contact and non-contact dispensing
    f)Disposable and fixed tips compare
    Self enrolment
  • This courses give the student an understanding of our products , services, and trainings provided.

    Self enrolment
    • Familiarize you with some terminology that you learned from our pre-req Basic Liquid Handling Bootcamp
    • Introduce you to methods for testing/calibration of automated liquid handling
    • Discuss “optimal” pipetting techniques
    • Introduction to calibration & validation/QC testing
    • Learn about different calibration methods
    • Hands on operation on real liquid handling robot(s) with liquid classes and volume deliverables per training agreement using a calibration kit with recommended Good Liquid Handling® settings
    • Empower you to calibrate an ALH
    Self enrolment