Liquid Handling Training Theory and Practice Workshop
- Teacher: Petar Stojadinovic
- Teacher: William Herms
- Teacher: Jason Dickman
Quick summary of topics:
Terminology
pipetting techniquesLow volume dry plate pipetting.
· Volume calibration.
· Setting up liquid classes.
· How to reduce liquid retention in tips when using the same tips for
multiple plates.
· Multi-pipetting (e.g. take up 15µl and dispense 5µl to all plates
accurately).
· How to mix properly after pipettingWash Cycles
Dilution Effects with Buffers
reagent and chemical compatibilityOverview of Validation and QC
Color DyesDecontamination
Goals of the course:
- Familiarize you with the terminology and methods of automated liquid handling
- Discuss “optimal” pipetting techniques
- Learn about reagent and chemical compatibility issues
- Introduction to validation/QC testing options
- Review proper decontamination techniques
- Try hands-on operations on real liquid handling robots with an aqueous liquid class using a calibration kit modifying aspiration and dispense speeds with contact and non-contact dispensing handling “issues” (the majority of the class)!
Full Summary of Course Topics:
1.Introduction to liquid handling robots for HTSa.Fixed tips versus disposable tips2.Liquid handling terminology, issues and techniques3.Reagent and chemical compatibility and handling issues4.Dilution Effects in Assay Development5.Validation and QC techniques and options6.Decontamination protocols7.Hands-on pipetting on real robots modifying speed in delivering a Standard aqueous liquid class solution colored with scientific dye(s)a)Measuring CV precisionb)Measuring CV accuracyc)Varying dispensing speedd)Varying Aspiratione)For contact and non-contact dispensingf)Disposable and fixed tips compareGood Pipetting Sales Training
- Teacher: Swetha Raghavendra
- Teacher: Kane Fong
- Teacher: faye afshari
This courses give the student an understanding of our products , services, and trainings provided.
- Familiarize you with some terminology that you learned from our pre-req Basic Liquid Handling Bootcamp
- Introduce you to methods for testing/calibration of automated liquid handling
- Discuss “optimal” pipetting techniques
- Introduction to calibration & validation/QC testing
- Learn about different calibration methods
- Hands on operation on real liquid handling robot(s) with liquid classes and volume deliverables per training agreement using a calibration kit with recommended Good Liquid Handling® settings
- Empower you to calibrate an ALH