Liquid Handling Training Theory and Practice Workshop
(Liquidhandling Pittcon)

Quick summary of topics:

Terminology
pipetting techniques

Low volume dry plate pipetting.
· Volume calibration.
· Setting up liquid classes.
· How to reduce liquid retention in tips when using the same tips for
multiple plates.
· Multi-pipetting (e.g. take up 15µl and dispense 5µl to all plates
accurately).
· How to mix properly after pipetting

Wash Cycles
Dilution Effects with Buffers
reagent and chemical compatibility

Overview of Validation and QC
Color Dyes

Decontamination

Goals of the course:

  • Familiarize you with the terminology and methods of automated liquid handling
  • Discuss “optimal” pipetting techniques
  • Learn about reagent and chemical compatibility issues
  • Introduction to validation/QC testing options
  • Review proper decontamination techniques
  • Try hands-on operations on real liquid handling robots with an aqueous liquid class using a calibration kit modifying aspiration and dispense speeds with contact and non-contact dispensing handling “issues” (the majority of the class)!

Full Summary of Course Topics:

1.Introduction to liquid handling robots for HTS
a.Fixed tips versus disposable tips
2.Liquid handling terminology, issues and techniques
3.Reagent and chemical compatibility and handling issues
4.Dilution Effects in Assay Development
5.Validation and QC techniques and options
6.Decontamination protocols
7.Hands-on pipetting on real robots modifying speed in delivering a Standard aqueous liquid class solution colored with scientific dye(s)
a)Measuring CV precision
b)Measuring CV accuracy
c)Varying dispensing speed
d)Varying Aspiration
e)For contact and non-contact dispensing
f)Disposable and fixed tips compare